SR5 from Trio can interact with Arf6. COS7 cells were transfected with HA-tagged Arf6 with or without FLAG-tagged EFA6. Cell extracts were incubated with GST-tagged TrioSR5-6 or TrioSR1. Complexes were immobilized on glutathione-Sepharose, extensively washed, and separated by SDS-PAGE together with aliquots of total cell lysates. Detection of bound protein was performed by immunoblotting with anti-HA antibody. The bound GST fusion proteins were visualized by Coomassie staining. Graph summarizes data for 3 independent experiments (mean ± s.d.); **p < 0.01 versus Arf6 and Trio SR5-6 (one way ANOVA).
Koo et al. BMC Cell Biology 2007 8:29 doi:10.1186/1471-2121-8-29