Dvl signals through two parallel pathways to regulate microtubule stability by Dvl. A) Neurons expressing Dvl an Gsk3β and treated with JNK inhibitor lose the residual level of MTs when compare with neurons expressing Dvl and Gsk3β. Scale bar 15 μm. B) Quantification shows that neurons expressing Dvl and Gsk3β and treated with JNK inhibitor have a decreased level of MTs stability than untreated neurons expressing Dvl and Gsk3β. C) Quantification shows that neurons expressing Dvl together with Gsk3β and treated with Anisomicin have comparable level of MT stability as untreated neurons. D) Inhibition of JNK by expression of JBD does not affect the level of phosphorylated MAP1B in granule cell neurons expressing Dvl when compared to control neurons expressing Dvl alone (arrow). Scale bar 10 μm. Two asterisks, P < 0.01; Three asterisks, P < 0.001.
Ciani and Salinas BMC Cell Biology 2007 8:27 doi:10.1186/1471-2121-8-27