Activation of JNK induces MT stability while inhibition of JNK blocks MT stability by Dvl. A) EGFP-expressing control neurons treated with low concentration of Anisomicin show higher level of microtubule stability when compared to EGFP-expressing neurons. Neurons expressing JNK alone reveals a level of stability comparable to that one obtained after exposure of neurons to Anisomicin. B) Quantification shows that Anisomicin treatment and JNK expression increase the level of MT stability in neurons C) Dvl-expressing neurons treated with the JNK inhibitor SP600125 or expressing the dominant negative JNK (JBD) show decreased MT stability when compared to control Dvl-expressing neurons. D) Quantification shows that treatment of Dvl expressing neurons with JNK inhibitor SP 600125 or expression of JBD reduces the level of MT stability. Scale bar 15 μm. Three asterisks, P < 0.001; For asterisks, P < 0.0001.
Ciani and Salinas BMC Cell Biology 2007 8:27 doi:10.1186/1471-2121-8-27