Induction of ATM activation in A549 cells by CS as revealed by Western blotting. The cells were exposed to 5, 10, 15 or 20 min of IM16 smoke (CS) and harvested at 30 min post-exposure. The extent of ATM activation (ATM-S1981P) was determined by Western blotting and compared with the respective samples of the mock treated cells (M) and with expression of ATM detected by antibody that was not phospho-specific.
Tanaka et al. BMC Cell Biology 2007 8:26 doi:10.1186/1471-2121-8-26