Streaming defect in gxcDD- cells. (A) AX2 and gxcDD- cells were allowed to starve on plastic plates. Images were continuously recorded. Streams formed in gxcDD- cells broke down to aggregates. (B) Streaming was also recorded in wild type and gxcDD- cells using an exogenous source of cAMP in a capillary chemotaxis assay. gxcDD- cells did migrate towards the cAMP source but did not form streams. (C) gxcDD- and AX2 cells were allowed to starve in Soerensen buffer at a density of 1 × 107 cells/ml and samples were withdrawn at the indicated times. The percentage of aggregated cells was determined by measuring the OD600. The data represent the average of three independent experiments.
Mondal et al. BMC Cell Biology 2007 8:23 doi:10.1186/1471-2121-8-23