Developmental delay in gxcDD- cells. (A) Parental wild-type AX2 and gxcDD- cells were allowed to develop on nutrient deficient agar plates. Images were recorded every 4 hrs. gxcDD- cells did develop to form fruiting bodies but were delayed in development by at least 4 hrs. (B) Cells were collected at 4 hr intervals for total protein and analysed by western blot. The appearance of the prespore marker pspA (detected by Mud1 monoclonal antibody) and the cell adhesion molecule csA (detected using monoclonal antibody 33-294-17) were monitored.
Mondal et al. BMC Cell Biology 2007 8:23 doi:10.1186/1471-2121-8-23