Association of GxcDD with Dictyostelium Rac GTPases. GST-RacGEF was expressed in E. coli and bound to glutathione-sepharose beads. Equal amounts of washed beads were incubated with cell lysates of AX2 cells expressing GFP fusions of 11 of 18 Dictyostelium Racs. Pulldown eluates were resolved by 12% SDS-PAGE and the proteins detected using a GFP monoclonal antibody.
Mondal et al. BMC Cell Biology 2007 8:23 doi:10.1186/1471-2121-8-23