Dominant-negative hook2 proteins do not alter the biochemical properties of CFTR. (A) Western blots indicate that co-expression of hook2 truncations did not significantly change the ratio of CFTR in the high (**) to low-molecular mass (*) bands or the detergent solubility of ΔF508-CFTR. (B) Western blots of homogenates of cells co-transfected with CFTR and various hook proteins or controls showed that steady state levels of wt CFTR or ΔF508-CFTR were not altered by the indicated hook proteins. Band C is the mature CFTR protein and band B the core glycosylated form . Equivalent loading was assured by evaluation of protein concentrations in cell lysates using the DC Protein Assay from BioRad. 20 μg total protein was loaded per well.
Szebenyi et al. BMC Cell Biology 2007 8:19 doi:10.1186/1471-2121-8-19