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Open Access Highly Accessed Research article

Phospholipase C and myosin light chain kinase inhibition define a common step in actin regulation during cytokinesis

Raymond Wong12, Lacramioara Fabian13, Arthur Forer3 and Julie A Brill12*

Author Affiliations

1 Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, TMDT Building, East Tower, 101 College St., Rm. 13-307, Toronto, Ontario M5G 1L7, Canada

2 Institute of Medical Science, University of Toronto, Toronto, Ontario, M5S 1A8, Canada

3 Department of Biology, York University, Toronto, Ontario M3J 1P3, Canada

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BMC Cell Biology 2007, 8:15  doi:10.1186/1471-2121-8-15

Published: 17 May 2007

Additional files

Additional file 1:

Meiosis in a crane-fly spermatocyte. Time-lapse phase-contrast micrographs of a dividing crane-fly primary spermatocyte. Times are hr:min:s. Movie corresponds to Figure 2A.

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Additional file 2:

Meiosis in a Drosophila spermatocyte. Time-lapse phase-contrast micrographs of a dividing Drosophila primary spermatocyte. Mitochondria and parafusorial membranes appear dark and outline the spindle during meiosis. Times are hr:min:s:ms. Movie corresponds to Figure 2B.

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Additional file 3:

Inhibition of PLC with U73122 causes cleavage furrow regression in a dividing crane-fly spermatocyte. Dividing crane-fly spermatocyte treated with 4.3 μM U73122 at 1 min 36 sec (14:51:00). Note that this cell was followed for only a short time after treatment. Times are hr:min:s. Movie corresponds to Figure 2C.

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Additional file 4:

Inhibition of PLC with U73122 causes cleavage furrow regression in a dividing Drosophila spermatocyte. Dividing Drosophila spermatocyte treated with 10 μM U73122 at 3 min 20 sec and washed at 18 min 20 sec. Times are hr:min:s:ms. Movie corresponds to Figure 2D.

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Additional file 5:

Inhibition of PLC with ET-18-OCH3 causes cleavage furrow regression in a dividing crane-fly spermatocyte. Dividing crane-fly spermatocyte treated with 35 μM ET-18-OCH3 at 13:32:00. Times are hr:min:s. Movie corresponds to Figure 3A.

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Additional file 6:

Inhibition of PLC with ET-18-OCH3 causes cleavage furrow regression in a dividing Drosophila spermatocyte. Dividing Drosophila spermatocyte treated with 35 μM ET-18-OCH3 at 5 min. 35 sec. Times are hr:min:s:ms. Movie corresponds to Figure 3B.

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Additional file 7:

The alkylating agent NEM inhibits cytokinesis in a dividing crane-fly spermatocyte. Dividing crane-fly spermatocyte treated with 1 mM NEM at 15:02:00. Times are hr:min:s. Movie corresponds to Figure 4A.

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Additional file 8:

The alkylating agent NEM inhibits cytokinesis in a dividing Drosophila spermatocyte. Dividing Drosophila spermatocyte treated with 1 μM NEM at 2 min 21 sec. Times are hr:min:s:ms. Cell treated similarly is shown in Figure 4B.

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Additional file 9:

Inhibition of MLCK with ML-7 causes cleavage furrow regression in a dividing Drosophila spermatocyte. Dividing Drosophila spermatocyte treated with 80 μM ML-7 at 3 min 30 sec. After the cell is washed at 4 min 55 sec, furrowing resumes. Times are hr:min:s:ms. Cell treated similarly is shown in Figure 5A.

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