Effects of SFLLR-NH2 (SFL, μM) and RLLFS-NH2 (RLL, μM) on release of MMP-2 and MMP-9 activities in HDFs. The cells were incubated with SFL, RLL or PAR-1 blocking antibody (BA, μg/ml) at 37°C for 6 h, respectively. Gelatin zymography was used to detect proMMP-2 and proMMP-9 activities. (A) MMP-2 and MMP-9 activities released from HDFs in response to various concentrations of SFL and TGFβ at 10 ng/ml (as a positive control); (B) MMP-2 and MMP-9 activities released from HDFs in response to SFL, RLL and BA. The values shown are Mean ± SD for four separate experiments. Cells from each one of the four dermal fibroblast donors were used for one independent experiment. * P < 0.05 in comparison with the response to medium alone (Con); † P < 0.05 in comparison with the response to the corresponding stimulus alone.
Wang et al. BMC Cell Biology 2007 8:14 doi:10.1186/1471-2121-8-14