Alterations in cellular and mitochondrial morphology induced by tBHP. (A) DIC images from untreated cells, showing normal membrane morphology. (B) Laser scanning confocal microscopy image from untreated cells showing triple labeling with Hoechst 33342 (blue), calcein (green) and TMRM (red). (C, D) Laser scanning confocal microscopy showing the fluorescence of the three dyes followed with the time, (C) 45 min, and (D) 90 minutes, after treatment with 80 μM tBHP. (E) Magnification of the cell indicated by an arrow in panel C and (F) magnification of the cell indicated by an arrow in panel D. (G) DIC images from H9c2 cells treated with 25 μM tBHP for three hours. (H) Laser scanning confocal microscopy (LSCM) image from cells treated with 25 μM tBHP during three hours. Images are representative from 3 different cell preparations. Panels B-D and G, H are the same magnification. Scale bar corresponds to 20 μm.
Sardão et al. BMC Cell Biology 2007 8:11 doi:10.1186/1471-2121-8-11