The dominant negative IκB mutant protein stimulates the dexamethasone induced downregulation of IAPs: MCF-7 parental cells and Clone 1 of the dnIκB expressing cells were treated for 20 min with TNF-α (10 ng ml-1) alone (A and C) or in combination with Dex (10 μM) (B and D): Protein expression of XIAP (A and B) or c-IAP (C and D) at different times was determined by Western blot: Actin was used as loading control. Each blot is representative of three independent experiments. Below the blots the bar graphs indicate the relative density of each lane with respect to the respective control, which has an arbitrary value of 1.
Machuca et al. BMC Cell Biology 2006 7:9 doi:10.1186/1471-2121-7-9