Dexamethasone inhibits in a dose-dependent manner the TNF-α mediated cytotoxicity in MCF-7 cells: (A) MCF-7 cells were treated with different concentrations of TNF-α and cell survival was determined at the indicated times (24, 48, 72 and 96 h). Values are mean ± SD from three independent experiments performed in triplicate. a indicates p < 0.001 with respect to control value. b indicates p < 0.001 with respect to the other values of the same group: (B) Cell survival of cultures treated with TNF-α (10 ng ml-1) for 48 h in the presence of different concentrations of Dex. Values are mean ± SD from three independent experiments performed in triplicate. * indicates p < 0.05 vs TNF + vehicle, ** indicates p < 0.001 vs. TNF + vehicle.: (C) Micrograph of sub-confluent MCF-7 cells treated for 48 h with 10 ng ml-1 of TNF-α in the presence (right panel) or absence of Dex 10 μM (left panel) (Scale bar: 25 μm).
Machuca et al. BMC Cell Biology 2006 7:9 doi:10.1186/1471-2121-7-9