Cadmium and arsenite activate JNK, p38 MAPK, and ERK in S2 cells. Four million cells were plated in 1 ml DSFM per well on 6-well plates 2 days before treatments. Cells were left untreated (Co) or received 10 μM or 100 μM CdCl2 (A), or 50 μM or 200 μM NaAsO2 (B). Treatments were done in duplicates. Cells were harvested at the indicated times after the treatments and analyzed for MAP kinase phosphorylation in immunoblot analyses using phosphoepitope-specific antibodies as indicated. Immunoblotblot analysis using an antibody recognizing JNK indicates equal protein loading. An experiment representative of 3 repetitions is shown.
Ryabinina et al. BMC Cell Biology 2006 7:7 doi:10.1186/1471-2121-7-7