Nuclear envelope transmembrane proteins (NETs) that are up-regulated during myogenesis

I-Hsiung Brandon Chen , Michael Huber , Tinglu Guan , Anja Bubeck and Larry Gerace

Department of Cell Biology, The Scripps Research Institute, 10555 N. Torrey Pines Rd., La Jolla CA 92037, USA

author email corresponding author email

BMC Cell Biology 2006, 7:38doi:10.1186/1471-2121-7-38


Published:	24 October 2006

Additional files

Additional File 1:

NETs and control genes examined by microarray and RT-PCR analysis. List of all genes analyzed in this study. This includes an updated list of NETs originally described in Ref. [22] together with gene annotations from standard BLAST analysis, a list of primers used for quantitative RT-PCR, and the probesets on Affymetrix Mouse Genome 430 2.0™ array that were recognized by the specific genes indicated

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Additional File 2:

Relative levels of NE markers and desmin at three developmental stages in 10 mouse tissues. Gene expression levels of selected lamina proteins were measured by quantitative real-time PCR in ten C129 mouse tissues from embryonic day 17 (E7), post-natal day 7 (P7) and adult. All signal values were normalized to peptidylprolyl isomerase A (Ppia) as in Fig. 4. The Y-axis indicates the signal value of RNA transcript after normalization to Ppia. The error bars indicate the standard deviation.

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Additional File 3:

Relative levels of NETs up-regulated in C2C12 differentiation at three developmental stages in 10 mouse tissues. Gene expression levels of the 6 NETs up-regulated during C2C12 differentiation were measured by quantitative real-time PCR in ten C129 mouse tissues from embryonic day 17 (E7), post-natal day 7 (P7) and adult. All signal values were normalized to peptidylprolyl isomerase A (Ppia) as in Fig. 4. The Y-axis indicates the signal value of RNA transcript after normalization to Ppia. The error bars indicate the standard deviation.

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Additional File 4:

Expression range of selected lamina proteins and the up-regulated NETs. A box-and-whisker graph shows gene expression levels of selected lamina proteins and the NETs in all tested mouse tissues. The expression values measured by RT-PCR in 2 and 3 were plotted on a log base 10 scale. Boxes indicate the median, the lower quartile and upper quartile values. Whiskers indicate the range of data points from the highest to the lowest. See [63].

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