Anodic polarity of phospholipid rearrangement and PS externalization. Anodic, equatorial, and cathodic PS externalization responses for individual Jurkat cells exposed to 50, 3 ns, 6 MV/m pulses were extracted photometrically from microscope images by defining tangential regions of interest approximately 2 μm by 0.5 μm at 0°, 90°, and 180° around the circumference of a Jurkat T cell, with 0° at the anode pole. Results are shown for images captured at 7 s, before externalized PS has diffused laterally away from the translocation sites (a), and at 5 min, showing the overall increased FM1-43 fluorescence after the translocated PS has become distributed evenly around the cell (b). Each group of three bars (anode, equator, cathode) represents the data from a single cell [29 cells for (a); 33 cells for (b)]. The rightmost group of three bars in each graph represents the mean of all cells in the data sets. A similar pattern is observed with 4 ns pulses.
Vernier et al. BMC Cell Biology 2006 7:37 doi:10.1186/1471-2121-7-37