The effect of signal transduction inhibitors on EGF-induced inhibition of lactogenic differentiation. HC11-luci cells were induced to differentiate in DIP-induction media in the presence or absence of EGF (10 ng/ml). Inhibitors were added alone or in combination at the time of induction (LY294002 10 μM, SB203580 10 μM, Rapamycin 100 nM, PD98059 20 μM). Luciferase activity was determined 48 hours post-induction and normalized to protein concentration. *These values represent statistically significant difference (P value .0001) from the DIP+EGF.
Galbaugh et al. BMC Cell Biology 2006 7:34 doi:10.1186/1471-2121-7-34