Additional file 3.
GFP localization through a single, stimulated cell. An NIH3T3 culture was serum deprived, injected with the PH-AKT-GFP plasmid, and stimulated as above. This series of fluorescent confocal images (40X/1.25 objective) illustrates the appearance of cytoplasmic structures that are at times visible in these stimulated cultures.
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Yang et al. BMC Cell Biology 2006 7:33 doi:10.1186/1471-2121-7-33