Additional file 1.
The appearance of a stimulated compared to an unstimulated cell, low power. Two NIH3T3 cultures were serum deprived for 30 hrs, microinjected with the PH-AKT-GFP plasmid, and cultured for 14 hrs prior to stimulation of only one of the cultures with 10% serum 10 min prior to photography. This movie is a sequence of fluorescent confocal images beginning near the coverslip and extending through one cell from the stimulated culture, and one cell from the unstimulated culture. The distribution of the fluorescence at the plasma membrane and away from the interior of the cell is apparent in the stimulated cell (40× objective, 10 micron steps).
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Yang et al. BMC Cell Biology 2006 7:33 doi:10.1186/1471-2121-7-33