Rad26 responds to conditions that destabilize microtubules. A. rad26Δ cells were sensitive to 8 μg/ml MBC. Cultures of TE236 (rad26+) and TE257 (rad26Δ) were serially diluted onto YE5S and YE5S + MBC plates and grown for 4 days at 30°C. B. TBZ did not cause relocalization of Rad22-GFP. A culture of TE1239 (rad22-gfp) was split and then treated with 20 μg/ml TBZ for five hours, 7.5 μg/ml Phleomycin for two hours, or left untreated. Bars = Std dev C. Rad26-GFP accumulated in the cytoplasm following MBC, but not Phleomycin, treatment. TE236 (rad26+) and TE1197 (rad26-gfp) cells cultured in minimal medium (EMM) were left untreated or treated with 8 μg/ml MBC for 3 hours or 10 μg/ml Phleomycin for 4 hours, fixed with paraformaldehyde and processed for microscopy. The Rad26-GFP signal was similar in live cells (data not shown). Bar = 7 μm.
Baschal et al. BMC Cell Biology 2006 7:32 doi:10.1186/1471-2121-7-32