Additional File 1.

poster
play_button

The movies are viewable using QuickTime, which can be downloaded from Apple's QuickTime Site http://www.apple.com/quicktime/download/ webcite. 3D-image reconstructions of A) cytoplasmically injected fluorescent actin and B) CD-BODIPY visualised in living HeLa cells expressing fluorescent NPC (POM121-GFP). M1A shows a maximum-intensity projection of three-dimensional dataset rendered from a confocal stack recording of an H-PomGFP cell subjected to cytoplasmic microinjection of fluorescent actin just minutes before the experiment. In this overlay movie projection the actin channel is pseudo-colored red, whereas POM121-GFP is pseudo-colored green.

Format: MOV Size: 1.2MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

M√ľnter et al. BMC Cell Biology 2006 7:23   doi:10.1186/1471-2121-7-23