Table 2

Effect of hirudin on IL-8 release from A549 cells induced by thrombin

Compound (U/ml)

IL-8 released (pg/ml)

2 h

16 h


Medium alone

18.7 ± 1.6

64.8 ± 7.2

Thrombin 3

40.1 ± 11.1

181 ± 32

Hirudin 3

18.4 ± 1.7

52.8 ± 5.7

Thrombin 3 + Hirudin 3

21.9 ± 7.2*

73.9 ± 12.9*

Thrombin 10

65.3 ± 18.4

478 ± 131

Hirudin 10

18.9 ± 2.1

59.3 ± 12.6

Thrombin 10 + Hirudin 10

26.8 ± 6.7*

119 ± 16.6*


Data shown are mean ± SE for five separate experiments. Thrombin, hirudin, and thrombin with hirudin were added to cells for 2 h or 16 h. *P < 0.05 compared with the corresponding concentration of thrombin alone.

Wang et al. BMC Cell Biology 2006 7:22   doi:10.1186/1471-2121-7-22

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