The mRNA expression of hTERT, c-myc and PPARγ genes in normal human PBMCs treated with growth medium containing 10% NHS and 25-hydroxycholesterol (25OH-C; 1 μM) along with propranolol (PROP; 200 μM). Total cellular RNA was extracted at indicated time points (h-hours) and amplified by RT- PCR. (A) Representative agarose gel photographs showing ethidium bromide stained RT-PCR products of hTERT, c-myc, PPARγ and β2M genes. (B) The normalized mRNA expression of hTERT, c-myc and PPARγ genes obtained after analysis with SCION IMAGE software is expressed as percentage of that in control (0 h) cells. Each data point represents mean ± SD of three independent experiments.
Sikand et al. BMC Cell Biology 2006 7:2 doi:10.1186/1471-2121-7-2