Thalidomide blocks angiogenesis. Fourth day incubated chicken eggs were broken, entire egg contents were transferred into a sterile petri dish. In vascular bed, heart and blood capillary are visible on 0 hour panel (left). Next, the vascular bed was incubated with 100 μg/ml thalidomide disc, placed on the vascular bed for 12 hours. It is evident from the representative images that 100 μg/ml thalidomide retarded the growth of the blood capillaries at the edges of vascular bed. A 70% loss of blood vessels (inset on top of the panels) was estimated by counting the number of red pixels using an analytical module of Adobe Photoshop ver. 6.0.
Tamilarasan et al. BMC Cell Biology 2006 7:17 doi:10.1186/1471-2121-7-17