Topoisomerase II, but not NuMA, is also aberrantly localized following sanguinarine treatment. Cultures were fixed and double-labeled with antibodies to topoisomerase II (green), NuMA (red), and counterstained with Hoechst to visualize DNA (blue). DIC, differential interference microscopy. (a-d) Control culture, showing normal distribution of topoisomerase II and NuMA. Note variable labeling of nuclei with topoisomerase antibody; cytoplasmic labeling, when present, is homogeneous throughout the cell. In contrast, NuMA is much more uniform in its distribution throughout all nuclei. (e-h) Culture treated with 5 μM sanguinarine for 24 hours. Similar to cyclin D1, topoisomerase II is also redistributed into numerous cytoplasmic speckles (arrow). NuMA remains uniformly labeled in all nuclei.
Holy et al. BMC Cell Biology 2006 7:13 doi:10.1186/1471-2121-7-13