Open Access Highly Accessed Research article

Microtubules regulate disassembly of epithelial apical junctions

Andrei I Ivanov*, Ingrid C McCall, Brian Babbin, Stanislav N Samarin, Asma Nusrat and Charles A Parkos

Author Affiliations

Epithelial Pathobiology Research Unit, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30322, USA

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BMC Cell Biology 2006, 7:12  doi:10.1186/1471-2121-7-12

Published: 1 March 2006

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Additional File 1:

Effects of nocodazole and pacitaxel on apical microtubules in colonic epithelial cells. Polarized monolayers of SK-CO-15 cells were treated for 2 h with either nocodazole (30 μM) or pacitaxel (10 μM) or vehicle, and microtubules and apical junctions were visualized using antibodies against α-tubulin and β-catenin respectively. As can be seen, the microtubule-depolymerizing agent nocodazole causes disappearance of the tubulin filament meshwork at the level of the AJC, whereas microtubule-stabilizing drug pacitaxel dramatically increases the density of apical microtubules.

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