The experiments outlined in Fig. 6 revealed that CD11b expression in DMSO-treated HL60 cells is a multi-step process involving an intermediate "primed" state. (A,B). The "restimulated" (solid diamonds) and "twice restimulated" (solid circles) cells both showed a similar increased rate of CD11b expression (fraction of CD11bHigh cells) relative to the "native" (open triangles) or "untreated" (open squares) groups. (C,D). This accelerated kinetics was sustained for up to four days in DMSO-free medium but was gradually lost beyond four days. Duplicates are presented, where solid circles, open diamonds, open triangles, solid squares, and solid triangles (--) represent cells cultured for 1, 2(for C)/4(for D), 5, 6, and 7 days in control medium before restimulation with 0.8% DMSO, respectively. Open squares indicate cells of the native population that have never been previously treated with DMSO nor FACS sorted. Each data point represents a flow cytometry measurement obtained from 10,000 cells.
Chang et al. BMC Cell Biology 2006 7:11 doi:10.1186/1471-2121-7-11