Characterization of gana-1, a Caenorhabditis elegans gene encoding a single ortholog of vertebrate α-galactosidase and α-N-acetylgalactosaminidase
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Institute of Inherited Metabolic Disorders, Charles University, 1st Medical Faculty, Prague, Czech Republic
BMC Cell Biology 2005, 6:5 doi:10.1186/1471-2121-6-5Published: 27 January 2005
Structure of GANA-1 dimer. The color of the backbone represents differences of amino acids between GANA-1 and chicken NAGA. Blue color represents identical residues and orange stands for non-conservative changes. The colors from cyan to green represent different degrees of conservation. The surface of one monomer unit at the interface area is rendered with colors representing electrostatic potential. N-acetyl-D-galactosamine (inhibitor) is placed in the active site pocket of both monomer units (D-GalNAc arrowhead). K257 arrowhead depicts predicted dimerisation residue.
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Immunofluorescence detection of GANA-1::GFP in muscle cells. 3D volume rendered and animated image corresponding to Figure 5A
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