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Open Access Research article

Characterization of sub-nuclear changes in Caenorhabditis elegans embryos exposed to brief, intermediate and long-term anoxia to analyze anoxia-induced cell cycle arrest

Vinita A Hajeri, Jesus Trejo and Pamela A Padilla*

Author Affiliations

Department of Biological Sciences, University of North Texas, Denton, TX, 76203, USA

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BMC Cell Biology 2005, 6:47  doi:10.1186/1471-2121-6-47

Published: 20 December 2005

Additional files

Additional File 1:

Chromosomes of prophase nucleus of an embryo exposed to normoxia. Embryos were collected, and stained with DAPI and the mAb414 to recognize nuclear pore complex. Spinning disk confocal microscopy was used to obtain Z-stack images of the prophase nucleus. The movie (QuickTime Player 7.0.3) depicts a 9.2 μm region that includes the nucleus cross-sectioned every 0.2 μm for a total of 46 cross sections. DAPI and mAb414 images were merged to evaluate prophase chromosome location relative to the nuclear membrane.

Format: MOV Size: 189KB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data

Additional File 2:

Chromosomes of prophase nucleus of an embryo exposed 30 minutes of anoxia. Embryos were collected, and stained with DAPI and the mAb414 to recognize nuclear pore complex. Spinning disk confocal microscopy was used to obtain Z-stack images of the prophase nucleus. The movie (QuickTime Player 7.0.3) depicts a 9.2 μm region that includes the nucleus cross-sectioned every 0.1 μm for a total of 92 cross sections. DAPI and mAb414 images were merged to evaluate prophase chromosome location relative to the nuclear membrane.

Format: MOV Size: 917KB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data

Additional File 3:

Chromosomes of prophase nucleus of an embryo exposed 24 hours of anoxia. Embryos were collected, and stained with DAPI and the mAb414 to recognize nuclear pore complex. Spinning disk confocal microscopy was used to obtain Z-stack images of the prophase nucleus. The movie (QuickTime Player 7.0.3) depicts an 8.4 μm region that includes the nucleus cross-sectioned every 0.2 μm for a total of 42 cross sections. DAPI and mAb414 images were merged to evaluate prophase chromosome location relative to the nuclear membrane.

Format: MOV Size: 37KB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data

Additional File 4:

Chromosomes of prophase nucleus of an embryo exposed to normoxia. Embryos were collected, and stained with DAPI, mAb414 to recognize nuclear pore complex and anti-gamma tubulin to recognize the centrosome. Spinning disk confocal microscopy was used to obtain Z-stack images of the prophase nucleus. The movie (QuickTime Player 7.0.3) depicts a 10.0 μm region that includes the nucleus cross-sectioned every 0.2 μm for a total of 50 cross sections. DAPI, mAb414 and anti-gamma tubulin images were merged to evaluate prophase chromosome location relative to the nuclear membrane.

Format: MOV Size: 1.3MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data

Additional File 5:

Chromosomes of prophase nucleus of an embryo exposed 30 minutes of anoxia. Embryos were collected, and stained with DAPI, mAb414 to recognize nuclear pore complex and anti-gamma tubulin to recognize the centrosome. Spinning disk confocal microscopy was used to obtain Z-stack images of the prophase nucleus. The movie (QuickTime Player 7.0.3) depicts a 7.0 μm region that includes the nucleus cross-sectioned every 0.2 μm for a total of 35 cross sections. DAPI, mAb414 and anti-gamma tubulin images were merged to evaluate prophase chromosome location relative to the nuclear membrane.

Format: MOV Size: 692KB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data