CpnA is present throughout development. Wildtype Dictyostelium cells in starvation buffer were placed on filters to develop. At 5-hour intervals, protein and RNA samples were harvested. A) Whole cell protein samples (5 × 106 cells per lane) from each time point were analyzed by Western blot using a purified CpnA antibody (arrow points to the 66 kDa CpnA). B) RNA samples from each time point were used in RT-PCR to detect cpnA mRNA. RT-PCR without the RT was run as a control (no RT). C) Whole cell protein samples (5 × 106 cells per lane) from wildtype cells and cpnA- cells were analyzed by Western blot using the CpnA antisera (arrow points to the 66 kDa CpnA).
Damer et al. BMC Cell Biology 2005 6:46 doi:10.1186/1471-2121-6-46