Research article
Copine A, a calcium-dependent membrane-binding protein, transiently localizes to the plasma membrane and intracellular vacuoles in Dictyostelium
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* Corresponding author: Cynthia K Damer cydamer@vassar.edu
Biology Department, Vassar College, 124 Raymond Ave., Poughkeepsie, NY 12604
BMC Cell Biology 2005, 6:46 doi:10.1186/1471-2121-6-46
Published: 12 December 2005Additional files
A single cell expressing GFP-CpnA that has been starved for 5.5 hrs (same cell shown in Figure 4A). GFP-CpnA translocates from the cytosol to membranes and back to the cytosol once.
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A single cell expressing GFP-CpnA that has been starved for 5.5 hrs (same cell shown in Figure 4B). GFP-CpnA translocates from the cytosol to membranes and back to the cytosol twice.
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A single cell expressing GFP-CpnA that has been starved for 4 hrs (not shown in Figure 4). GFP-CpnA translocates from the cytosol to membranes and back to the cytosol six times.
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Adjacent cells expressing GFP-CpnA that have been starved for 10 hrs. GFP-CpnA translocates from the cytosol to membranes multiple times in each cell.
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A small aggregate of cells expressing GFP-CpnA that has been starved for 9.5 hrs (one of these cells is shown in Figure 4C). GFP-CpnA translocates from the cytosol to membranes and back to the cytosol once in four different cells at different times.
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Cells expressing GFP-CpnA were placed in water to promote bursting and imaged with a confocal microscope every 2.5 s (same cells as in Figure 5A).
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Cells expressing GFP-CpnA were placed in water containing 2 mM EGTA to chelate calcium and imaged with a confocal microscope every 2.5 s (same cells as in Fig. 5B).
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Cells expressing GFP alone were placed in water to promote bursting and imaged with a confocal microscope every 2.5 s (same cells as in Fig. 5C).
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A z-series of images taken from the bottom to the top of a single fixed cell expressing GFP-CpnA (same cell as in Fig. 6G).
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