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Open Access Highly Accessed Research article

Copine A, a calcium-dependent membrane-binding protein, transiently localizes to the plasma membrane and intracellular vacuoles in Dictyostelium

Cynthia K Damer*, Marina Bayeva, Emily S Hahn, Javier Rivera and Catherine I Socec

Author Affiliations

Biology Department, Vassar College, 124 Raymond Ave., Poughkeepsie, NY 12604

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BMC Cell Biology 2005, 6:46  doi:10.1186/1471-2121-6-46

Published: 12 December 2005

Additional files

Additional File 1:

A single cell expressing GFP-CpnA that has been starved for 5.5 hrs (same cell shown in Figure 4A). GFP-CpnA translocates from the cytosol to membranes and back to the cytosol once.

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Additional File 2:

A single cell expressing GFP-CpnA that has been starved for 5.5 hrs (same cell shown in Figure 4B). GFP-CpnA translocates from the cytosol to membranes and back to the cytosol twice.

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Additional File 3:

A single cell expressing GFP-CpnA that has been starved for 4 hrs (not shown in Figure 4). GFP-CpnA translocates from the cytosol to membranes and back to the cytosol six times.

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Additional File 4:

Adjacent cells expressing GFP-CpnA that have been starved for 10 hrs. GFP-CpnA translocates from the cytosol to membranes multiple times in each cell.

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Additional File 5:

A small aggregate of cells expressing GFP-CpnA that has been starved for 9.5 hrs (one of these cells is shown in Figure 4C). GFP-CpnA translocates from the cytosol to membranes and back to the cytosol once in four different cells at different times.

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Additional File 6:

Cells expressing GFP-CpnA were placed in water to promote bursting and imaged with a confocal microscope every 2.5 s (same cells as in Figure 5A).

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Additional File 7:

Cells expressing GFP-CpnA were placed in water containing 2 mM EGTA to chelate calcium and imaged with a confocal microscope every 2.5 s (same cells as in Fig. 5B).

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Additional File 8:

Cells expressing GFP alone were placed in water to promote bursting and imaged with a confocal microscope every 2.5 s (same cells as in Fig. 5C).

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Additional File 9:

A z-series of images taken from the bottom to the top of a single fixed cell expressing GFP-CpnA (same cell as in Fig. 6G).

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