Early developmental gene expression in AX2 and HSB61 cells. Total RNA was extracted from cells pulsed (+ cAMP) or not (- cAMP) in suspension for the time indicated. After electrophoresis and transfer, the membranes were hybridized with radiolabelled acaA, carA, csA. Actin was used as control.
Arigoni et al. BMC Cell Biology 2005 6:43 doi:10.1186/1471-2121-6-43