(a) Evolution of intensity of dimerized receptors with two ligands (high intensity spots) and of monomer plus dimerized receptors with a single ligand bound (low intensity spots) along with the data of single particle tracking experiments by Sako et al. over time intervals of 20 sec. The simulations were performed for a receptor number density of 5500 per μm2, a diffusivity of D = 2 × 10-14 m2sec-1, and 18% dimers initially. The simulation intensity has been normalized with the experimental data. (b) Comparison of predicted density of Cy3-EGF spots with experimental data of Sako et al. The densities are normalized with the value at 60 sec. Good agreement of simulations with experimental data is found. In both panels, error bars indicate 2 standard deviations obtained from 10 independent MC simulations.
Mayawala et al. BMC Cell Biology 2005 6:41 doi:10.1186/1471-2121-6-41