Expression of a GFP-MFP fusion protein in onion epidermal cells labels peroxisome-like structures and MT-like filaments. Onion epidermal layers were bombarded with DNA constructs encoding either GFP-MFP (A), GFP containing a carboxyl-terminal PTS1 (GFP-SRM) (B), or GFP alone (C), and visualized by epifluorescence microscopy. The inset in (A) shows a representative confocal image of peroxisome-like structures and MT-like filaments in the same optical Z-section in the cortical region of a GFP-MFP-expressing cell. Bars, 20 μm and 3 μm (A, inset).
Chuong et al. BMC Cell Biology 2005 6:40 doi:10.1186/1471-2121-6-40