Research article
The peroxisomal multifunctional protein interacts with cortical microtubules in plant cells
1 Department of Biological Sciences, University of Calgary, 2500 University Dr. NW, Calgary, AB, Canada T2N 1N4, Canada
2 Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada N1G 2W1, Canada
BMC Cell Biology 2005, 6:40 doi:10.1186/1471-2121-6-40
Published: 28 November 2005Additional files
Movie1.mov – Real-time image sequence of an onion epidermal cell expressing GFP-MFP shows apparent interactions between peroxisomes and cortical MTs. Epifluorescence images (n = 200) were captured every 0.13 seconds. A mobile, dumbbell-shaped peroxisome (tracked with the green marker) appears to interact with MTs at several locations during the initial part of the image sequence before moving out of focus. Upon returning into the focal plane near the end of the image sequence, this peroxisome again appears to interact with MTs. The movement of this peroxisome is also shown in the fixed image sequence in Figure 6. Another peroxisome demonstrated a pausing event at a location where MTs were absent (tracked with the red marker), although it also paused several times at locations that coincided with MTs. The peroxisome tracked with the blue marker demonstrated on two occasions a subtle, lateral gliding movement alongside MTs. The cell observed in this image sequence was from an unpeeled epidermal layer that remained associated with the leaf segment.
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Movie2.mov – Real-time image sequence of an onion epidermal cell expressing GFP-MFP that demonstrated a lower frequency of peroxisome pausing events at sites that were coincident with MT locations. Epifluorescence images (n = 270) were captured every 0.2 seconds.
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Movie3.mov – Real-time image sequence of an oryzalin treated onion epidermal cell expressing GFP-MFP. This movie demonstrates that MT depolymerization does not result in an obvious reduction of peroxisome pausing events in cell cortex. Epifluorescence images (n = 150) were collected every 0.2 seconds.
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