Changes in LPL mass and activity during incubation of adipose tissue explants. Epididymal adipose tissue was dissected from fed and 24 h-fasted rats, cut into small pieces and rinsed in cold PBS. About 100 mg tissue was then incubated at 37°C for the indicated times as described in the Methods section. LPL mass (triangles, panel A) and activity (circles, panel B) in tissue explants from fed (filled symbols, ● and ▼) or fasted (open symbols, ○ and ▽) rats. Values at the start of the incubations were for LPL mass: 17.4 ± 1.1 and 17.1 ± 2.5 ng/μg DNA and for LPL activity: 6.3 ± 1.8 and 3.5 ± 0.7 mU/μg DNA, in tissues from fed and fasted rats, respectively. The inset shows a separate experiment where incubations were stopped at shorter times. Only LPL mass was followed in that experiment. Some of the data points for fed and fasted rats fall on top of each other. Values are mean ± SEM for five parallel incubations.
Wu et al. BMC Cell Biology 2005 6:4 doi:10.1186/1471-2121-6-4