Table 1 |
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Summmary Of Granulocyte Immunochemistry*. |
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|
ANTIBODY |
HUMAN BLOOD GRANULOCYTES |
MOUSE BLOOD GRANULOCYTES |
MOUSE MPRO CELLS** |
|
|
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|
dimethyl H3K9 |
+ N |
+ N |
+ N |
|
trimethyl H3K9 |
+ N g |
+ N p |
+ N p |
|
monomethyl H3K27 |
+ N |
+ N |
+ N |
|
dimethyl H3K27 |
+ N |
+ N |
+ N |
|
trimethyl H3K27 |
+ N |
+ N |
+ N |
|
dimethyl H4K20 |
+/- N, C |
+/- N |
+/- N |
|
trimethyl H4K20 |
+ N, C |
+ N p |
+ N p |
|
HP1 α |
0‡ |
0 |
+/- N, C |
|
HP1 β |
0‡ |
0 |
+/- N, C |
|
HP1 γ |
+/- N, C |
+ N p |
+ N p |
|
|
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*The results listed represent a qualitative assessment, combining data from immunostaining and immunoblotting. In the case of normal mouse blood, due to inadequate enrichment of granulocytes, no immunoblotting was performed. ** Identical immunostaining and immunoblotting results were obtained with undifferentiated and RA differentiated MPRO cells. Abbreviations: N, nuclear staining; N g, nuclear coarse granules; N p, nuclear pericentric heterochromatin; N, C, nuclear and cytoplasmic staining. Reaction intensity: +, moderate to strong; +/-, weak; 0, negligible. ‡ Moderate to strong staining in the cytoplasm only. |
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Olins and Olins BMC Cell Biology 2005 6:39 doi:10.1186/1471-2121-6-39 |
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