Table 1

Summmary Of Granulocyte Immunochemistry*.

ANTIBODY

HUMAN BLOOD GRANULOCYTES

MOUSE BLOOD GRANULOCYTES

MOUSE MPRO CELLS**


dimethyl H3K9

+ N

+ N

+ N

trimethyl H3K9

+ N g

+ N p

+ N p

monomethyl H3K27

+ N

+ N

+ N

dimethyl H3K27

+ N

+ N

+ N

trimethyl H3K27

+ N

+ N

+ N

dimethyl H4K20

+/- N, C

+/- N

+/- N

trimethyl H4K20

+ N, C

+ N p

+ N p

HP1 α

0

0

+/- N, C

HP1 β

0

0

+/- N, C

HP1 γ

+/- N, C

+ N p

+ N p


*The results listed represent a qualitative assessment, combining data from immunostaining and immunoblotting. In the case of normal mouse blood, due to inadequate enrichment of granulocytes, no immunoblotting was performed.

** Identical immunostaining and immunoblotting results were obtained with undifferentiated and RA differentiated MPRO cells.

    Abbreviations
: N, nuclear staining; N g, nuclear coarse granules; N p, nuclear pericentric heterochromatin; N, C, nuclear and cytoplasmic staining.

    Reaction intensity
: +, moderate to strong; +/-, weak; 0, negligible.

Moderate to strong staining in the cytoplasm only.

Olins and Olins BMC Cell Biology 2005 6:39   doi:10.1186/1471-2121-6-39

Open Data