Eps8-induced actin remodeling in Xenopus embryos. Distribution of actin (A,C,D,E,G,H) revealed by phalloidin staining and Eps8-myc (B,D,F,H) revealed by 9e10 anti-c-myc antibody in animal cap cells of Xenopus embryos. In control animal caps, actin filaments are enriched at cell-cell junctions in superficial epithelial cells (A) and the cortex of deep cells (E). (B-D) In superficial epithelial cells, Eps8 expression causes an enrichment of actin filaments at cell-cell junctions (arrowheads). (F-H) In deep cells facing the blastocoel, Eps8 expression induces the formation of actin cables (arrowheads). (D,H) Eps8 is red and actin is green in merged images. Scale bar is equal to 10 μm.
Roffers-Agarwal et al. BMC Cell Biology 2005 6:36 doi:10.1186/1471-2121-6-36