KBP is essential for a regular distribution of mitochondria in NIH3T3 cells. A, a scheme of the anti-KBP plasmid is depicted. The reduction of endogenous KBP in 293 cells after transient transfection with the anti-KBP plasmid is shown. (B, C) NIH3T3 cells were transiently transfected with anti-sense KBP and 14-3-3γ-HA constructs in a 10:1 ratio, respectively, and costained with HA monoclonal antibody (B), and MitoTracker (C). As a control, NIH3T3 cells were transiently transfected with 14-3-3γ-HA only (D, E). Similar as in B-E, cotransfection with GFP was performed (F-I). Alternatively, anti-sense KBP transfected cells were stained with antibodies against β-tubulin (J) and with MitoTracker (K). Arrows in J, K indicate transfected cells. The merged picture of J and K is shown in (L). Trf, transfection; bars, 20 μm.
Wozniak et al. BMC Cell Biology 2005 6:35 doi:10.1186/1471-2121-6-35