Figure 4.

Immunoelectron micrographs demonstrating no co-localization of GFP-HPS3-delCBD and clathrin. Double-labeling of anti-clathrin (10-nm gold) and anti-GFP (15-nm gold) in normal melanocytes electroporated with GFP-HPS3-delCBD [A-D]. GFP-HPS3-delCBD (arrowheads) was largely cytoplasmic and distributed throughout the entire cell, from the perinuclear/Golgi region [A,B,D] to the tips [C]. No co-localization of the two labels (GFP-HPS3-delCBD and clathrin) was observed. N=Nucleus, G=Golgi, E=Endosomal structure, M=Melanosome. Bar = 500 nm

Helip-Wooley et al. BMC Cell Biology 2005 6:33   doi:10.1186/1471-2121-6-33
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