Table 1

GAGs effects on C6 Glioma detachment

Substrate

GAG

% Detachment


Type IV Collagen

Heparin

0% ± 0.14

Chondroitin Sulfate

7.5 % ± 0.7

Dextran Sulfate

0% ± 0.2

Fibronectin

Heparin

5% ± 0.9

Chondroitin Sulfate

4% ± 0.6

Dextran Sulfate

0% ± 0.2

Laminin

Heparin

0% ± 0.9

Chondroitin Sulfate

0% ± 0.12

Dextran Sulfate

0% ± 0.5

Culture dish plastic

Heparin

7% ± 0,2

Chondroitin Sulfate

5% ± 0,1

Dextran Sulfate

2% ± 0,3

BSA

Heparin

0% ± 0.1

Chondroitin Sulfate

5% ± 0,7

Dextran Sulfate

4% ± 0,4


Wells were coated with 10 μg/ml of type IV collagen, Fibronectin or Laminin. Detachment assays were performed as described. Results were compared with cells seeded on ECM substrate only. Coating with BSA or the culture dish plate was used as controls. Each point represents the mean of three independent experiments performed in triplicate ± SD.

Mendes de Aguiar et al. BMC Cell Biology 2005 6:31   doi:10.1186/1471-2121-6-31

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