Syntaxin 2 and 3 staining coincides with actin at cortical regions. The NRK cells were fixed, permeabilized and then double stained with syntaxin 2 (A) or 3 (C) anti-serum, LRSC-conjugated goat anti-rabbit IgG as well as guinea pig anti-α-ADF (B,D) and FITC-conjugated donkey anti-guinea pig IgG. Conventional fluorescence images were viewed using an Olympus AX70 fluorescence microscope. Bars, 10 μm.
Band and Kuismanen BMC Cell Biology 2005 6:26 doi:10.1186/1471-2121-6-26