Table 2 |
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|
Effects of GSNO treatment on agonist (CCh) dose-response parameters in [35S]GTPγS binding assays of hM4 cell line membranes. Membranes were preincubated in control conditions or in the presence of 0.5 mM GSNO for 30 min. Values are mean ± SE from three to four independent experiments performed in duplicate. Emax is expressed in percentage over basal with nonspecific binding subtracted. |
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|
Control |
GSNO |
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|
|
|
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|
CHO cell line |
log(EC50) |
Emax (%) |
log(EC50) |
Emax (%) |
|
|
||||
|
hM4-WT-A1 |
-4.87 ± 0.14 |
586 ± 31 |
-4.94 ± 0.09 |
674 ± 23 |
|
hM4-WT-E5 |
-4.87 ± 0.05 |
213 ± 3 |
-4.98 ± 0.13 |
276 ± 10** |
|
hM4-WT-C2 |
-5.21 ± 0.20 |
134 ± 3 |
-5.27 ± 0.20 |
154 ± 8* |
|
hM4-C133S-H2 |
-5.13 ± 0.10 |
198 ± 4 |
-5.12 ± 0.11 |
235 ± 6** |
|
|
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|
* Statistically different from control (P < 0.05) ** Statistically different from control (P < 0.01) |
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|
Kokkola et al. BMC Cell Biology 2005 6:21 doi:10.1186/1471-2121-6-21 |
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