Analysis of CRM1-dependent nuclear export of amino acid region 74 to 133 of 16.4.1. HeLa cells were transfected with plasmids directing expression of GFP fusion proteins containing 16.4.1 region 74–133 in single copy or in tandem (74–133)2. Representative images of the subcellular distribution of the GFP fusion proteins in untreated (-) and LMB treated cells (+) are shown at the top. Symbols in the graph indicate the nuclear proportion of fluorescence (%) in individual cells and horizontal lines and numbers the median of the cell population. Scale bars: 10 μm. Inhibition of CRM1 by LMB treatment increases nuclear proportion of GFP fusion proteins containing aa residues 74 to 133 of 16.4.1. GFP fusion proteins containing two copies of region 74 to 133 show stronger cytoplasmic localization than GFP fusion proteins with a single copy. These results indicate that region 74 to 133 of 16.4.1 is a substrate for CRM1-dependent export, which is recognized more efficiently in tandem than as a single copy.
Kramer-Hämmerle et al. BMC Cell Biology 2005 6:20 doi:10.1186/1471-2121-6-20