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Time-lapse motility and proliferation of KM101 and MG-63 cells. Images from four wells were combined to show the differential effects of laminin (upper left), collagen type I (upper right), collagen type IV (lower left) and mock-coated plastic (lower right). Red outlines indicate the perimeters of the cell "objects" from which object areas and centroid positions are derived. Green lines indicate tracks that are established between successive centroid positions that define the individual cell paths. In this image sequence, track segments are erased following 10 hours elapsed time. The total elapsed time of 114 hours for each cell type shows cells from shortly after seeding to near confluence. Images were acquired at 30-minute intervals and are displayed in the video at a rate of 6 images per second.

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Bahnson et al. BMC Cell Biology 2005 6:19   doi:10.1186/1471-2121-6-19