Individual cell velocities over time. Instantaneous velocity for individual KM101 cells is plotted for each time point (30 minute intervals) across time periods that extend in each case from the initiation to the termination of individual cell tracks. Cell tracks from four wells with different surface coatings (Collagen I – blue, Collagen IV – red, Laminin – yellow, Plastic – green) are shown; coating concentrations were 100 ug/ml in each case. The number at the top of each frame indicates the "unique track index" by which the tracks are identified. Not all surface coatings are represented in each frame, because in many cases tracks that were initiated did not meet criteria for track length, fence size, or object area (see Methods). These plots show the magnitude of individual non-smoothed velocity vectors, indicating cell motion plus random contribution from stage noise and cell-outline error at each 30-minute time point. In many cases the same cell may terminate in one frame and reinitiate in another due to collision.
Bahnson et al. BMC Cell Biology 2005 6:19 doi:10.1186/1471-2121-6-19