Velocity histograms by manual and automated tracking. Velocity measurements were acquired through manual tracking (Panel A) or through automated tracking (Panel B) from image sets from four wells as described in the text. Different coating compounds are indicated along the y-axis (all 10 ug/ml). Red portions of the bars for automated tracking indicate measurements that came from tracks with fence sizes of less than 11 pixels (i.e. 20 um or less motion in the x or y direction over the length of the track). These tracks (red portions) were selected for exclusion based upon the supposition, supported by Figure 3, that such tracks arose predominantly from non-cell stationary objects. Note in particular how subtraction of the red portions from the 0.0, 0.1, and 0.2 um/min bins for Collagen IV and Collagen I from the automated track histogram (panels B) would bring the remaining data (blue portions) more in line with the profile of data from manual tracks (Panel A).
Bahnson et al. BMC Cell Biology 2005 6:19 doi:10.1186/1471-2121-6-19