Open Access Highly Accessed Methodology article

Automated measurement of cell motility and proliferation

Alfred Bahnson1*, Charalambos Athanassiou1, Douglas Koebler1, Lei Qian1, Tongying Shun1, Donna Shields2, Hui Yu2, Hong Wang2, Julie Goff2, Tao Cheng2, Raymond Houck1 and Lex Cowsert1

Author Affiliations

1 Automated Cell, Inc. 390 William Pitt Way, Pittsburgh, PA, 15238 USA

2 University of Pittsburgh Cancer Institute, Research Pavilion at The Hillman Cancer Center, 5117 Center Ave, Pittsburgh, PA, 15213-1863 USA

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BMC Cell Biology 2005, 6:19  doi:10.1186/1471-2121-6-19

Published: 14 April 2005

Additional files

Additional File 1:

Time-lapse motility and proliferation of KM101 and MG-63 cells. Images from four wells were combined to show the differential effects of laminin (upper left), collagen type I (upper right), collagen type IV (lower left) and mock-coated plastic (lower right). Red outlines indicate the perimeters of the cell "objects" from which object areas and centroid positions are derived. Green lines indicate tracks that are established between successive centroid positions that define the individual cell paths. In this image sequence, track segments are erased following 10 hours elapsed time. The total elapsed time of 114 hours for each cell type shows cells from shortly after seeding to near confluence. Images were acquired at 30-minute intervals and are displayed in the video at a rate of 6 images per second.

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Additional File 2:

Acceleration of MG-63 cells. Time-lapse videos show MG-63 cells on laminin-coated (10 ug/ml, first image sequence "I04") and mock-coated plastic (second image sequence "J03"). Images were acquired at 30-minute intervals and are displayed in the video at a rate of 6 images per second. Green lines indicate automatically generated tracks connecting successive centroid positions of objects. Track segments are erased after 15 images, so the maximum track length represents 7.5 hours' migration. A total of 118 hours elapsed time is shown for each image sequence.

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Additional File 3:

Tracking scenarios. This tracking example sequence was taken from the set of images that are shown in the upper left panel of Movie 1 in order to illustrate in more detail how the tracking algorithm handles "scenarios" such as the colliding and splitting of cells. A total elapsed time of 35 hours (70 scans) is depicted, and specific details are given in the video to explain example scenarios. Original image quality is not preserved in the videos due to necessary compression.

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