Email updates

Keep up to date with the latest news and content from BMC Cell Biology and BioMed Central.

Open Access Research article

Trapping of normal EB1 ligands in aggresomes formed by an EB1 deletion mutant

Nick P Riess1, Kelly Milward1, Tracy Lee1, Matthew Adams2, Jon M Askham2 and Ewan E Morrison1*

Author Affiliations

1 CRUK Clinical Centre at Leeds, Division of Cancer Medicine Research, St James's University Hospital, Leeds LS9 7TF, UK

2 Molecular Medicine Unit, University of Leeds, Clinical Sciences Building, St. James's University Hospital, Leeds LS9 7TF, UK

For all author emails, please log on.

BMC Cell Biology 2005, 6:17  doi:10.1186/1471-2121-6-17

Published: 6 April 2005

Additional files

Additional File 2:

EB1-ΔN2-GFP aggregates in living COS-7 cells. Sequence 1: image capture rate was 1 frame/5s using a 40X lens, playback rate is 20 frames/s. The dynamic behaviour of EB1-ΔN2-GFP aggregates is again evident. Sequence 2: image capture rate was 1 frame/5s using a 40X lens, playback rate is 20 frames/s. This movie shows the cells in sequence 1 imaged by phase-contrast microscopy and allows the behaviour of these cells to be compared with two adjacent untransfected cells. The sequence begins 30s after the end of sequence 1. EB1-ΔN2-GFP aggregates are visible as phase-dense structures but their presence has no obvious effect upon cell behaviour.

Format: MOV Size: 5MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data

Additional File 1:

EB1-ΔN2-GFP aggregate morphology and dynamics. Image capture rate was 1 frame/5s using a 63X oil immersion lens, playback rate is 20 frames/s. Aggregates consist of brighter puncta in a matrix of lower fluorescence intensity. The dynamic nature of the aggregate can be seen. Structures can be observed extending away from the aggregate while remaining attached to it; on obvious example of this can be seen around the nuclear periphery at the bottom left of the aggregate.

Format: MOV Size: 530KB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data

Additional File 3:

Extrusion and retraction of a linear structure from an EB1-ΔN2-GFP aggregate. Image capture rate was 1 frame/5s using a 63X oil immersion lens, playback rate is 20 frames/s. Sequence 1: the extension of a linear fluorescent structure from an EB1-ΔN2-GFP aggregate is shown; the aggregate itself is overexposed to allow better resolution of this structure, which consists of brighter puncta linked to each other and the aggregate by a ribbon of less fluorescent material. Sequence 2: imaging was initiated approximately 1 min after the end of sequence 1. Retraction of the structure extended by the EB1-ΔN2-GFP aggregate can be seen.

Format: MOV Size: 2.4MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data

Additional File 4:

Movement of EB1-ΔN2-GFP aggregates is microtubule-dependent. Image capture rate was 1 frame/5s using a 40X lens, playback rate is 20 frames/s. This movie shows aggregate behaviour in cells after 2 h incubation in nocodazole. In the presence of the drug the aggregates are completely immobile.

Format: MOV Size: 1.7MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data

Additional File 5:

Perinuclear EB1-ΔN2-GFP aggregate assembly is microtubule-dependent. Image capture rate was 1 frame/10s using a 63X oil immersion lens, playback rate is 20 frames/s. Cells were incubated in nocodazole for 4 h followed by a wash and the addition of fresh imaging medium without drug. Imaging was initiated 30 min after nocodazole wash out. In the absence of drug EB1-ΔN2-GFP aggregates regain motility. Movement is intermittent and with an overall retrograde bias leading to the growth of perinuclear aggregates.

Format: MOV Size: 2MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data