Intracellular localization of recombinant Δ51CB. LCLC-103H cells with transient Δ51CB-EGFP expression revealed an intensive mitochondrial distribution of the fluorescence signals. Beside a slight cytoplasmic background there was also a signal level in the nucleoplasm, which reached ~40% of the mitochondrial fluorescence intensity. The nucleoli were lacking any fluorescence. A profile cut across the cell elucidates the intensity proportions. The arrow tips mark the nuclear boundary. The intensity gap within this region represents a transected nucleolus. For deconvolution, 23 optical sections were used, which have been acquired with a distance of 200 nm. WFM; obj. 63×/1.4 Oil.
Bestvater et al. BMC Cell Biology 2005 6:16 doi:10.1186/1471-2121-6-16